Co-Immunoprecipitation assay for HA-tagged proteins from yeast
نویسندگان
چکیده
منابع مشابه
Serial Immunoprecipitation of 3xFLAG/V5-tagged Yeast Proteins to Identify Specific Interactions with Chaperone Proteins
This method was generated to isolate high affinity protein complexes from yeast lysate by performing serial affinity purification of doubly tagged 3×FLAG/V5 proteins. First, the bait protein of interest is immunoprecipitated by anti-FLAG-conjugated magnetic beads and gently eluted by 3×FLAG antigen peptide. Next, the bait protein is recaptured from the first eluate by anti-V5-conjugated magneti...
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MOTIVATION Advanced technologies are producing large-scale protein-protein interaction data at an ever increasing pace. A fundamental challenge in analyzing these data is the inference of protein machineries. Previous methods for detecting protein complexes have been mainly based on analyzing binary protein-protein interaction data, ignoring the more involved co-complex relations obtained from ...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2019
ISSN: 2043-0116
DOI: 10.1038/protex.2019.004